Genetic Characterization of the Gypsy Moth from China (Lepidoptera, Lymantriidae) Using Inter Simple Sequence Repeats Markers
نویسندگان
چکیده
This study provides the first genetic characterization of the gypsy moth from China (Lymantriadispar), one of the most recognized pests of forests and ornamental trees in the world. We assessed genetic diversity and structure in eight geographic populations of gypsy moths from China using five polymorphic Inter simple sequence repeat markers, which produced reproducible banding patterns. We observed 102 polymorphic loci across the 176 individuals sampled. Overall genetic diversity (Nei's, H) was 0.2357, while the mean genetic diversity within geographic populations was 0.1845 ± 0.0150. The observed genetic distance among the eight populations ranged from 0.0432 to 0.1034. Clustering analysis (using an unweighted pair-group method with arithmetic mean and multidimensional scaling), revealed strong concordance between the strength of genetic relationships among populations and their geographic proximity. Analysis of molecular variance demonstrated that 25.43% of the total variability (F ST = 0.2543, P < 0.001) was attributable to variation among geographic populations. The results of our analyses investigating the degree of polymorphism, genetic diversity (Nei's and Shannon) and genetic structure, suggest that individuals from Hebei may be better able to adapt to different environments and to disperse to new habitats. This study provides crucial genetic information needed to assess the distribution and population dynamics of this important pest species of global concern.
منابع مشابه
DNA Barcoding of Gypsy Moths From China (Lepidoptera: Erebidae) Reveals New Haplotypes and Divergence Patterns Within Gypsy Moth Subspecies.
The gypsy moth from Asia (two subspecies) is considered a greater threat to North America than European gypsy moth, because of a broader host range and females being capable of flight. Variation within and among gypsy moths from China (nine locations), one of the native countries of Asian gypsy moth, were compared using DNA barcode sequences (658 bp of mtDNA cytochrome c oxidase subunit 1 [COI]...
متن کاملAttraction of the gypsy moth to volatile organic compounds (VOCs) of damaged Dahurian larch.
Olfactory responses of the gypsy moth Lymantria dispar (L.) (Lepidoptera: Lymantriidae), a major defoliator of deciduous trees, were examined in Inner Mongolia, China. We studied whether the gypsy moth adults are attracted by the major volatile organic compounds (VOCs) of damaged Larix gmelinii (Dahurian larch) foliage and compared the attractiveness of the plant volatiles with that of the synt...
متن کاملGenetic and Molecular Dissection of Blast Resistance in Rice Using RFLP, Simple Sequence Repeats and Defense-Related Candidate Gene Markers
Blast, Pyricularia grisea (Cooke) Sacc., is one of the most destructive diseases of rice worldwide and canresult in significant reductions in yield. The use of resistant cultivars is the most economical and effectiveway of controlling rice blast. A variety of DNA markers, including plant defense-related candidategene markers are available for genetic characterization and molec...
متن کاملIdentification and characterization of a RAPD-PCR marker for distinguishing Asian and North American gypsy moths.
The recent introduction of the Asian gypsy moth (Lymantria dispar L.) into North America has necessitated the development of genetic markers to distinguish Asian moths from the established North American population, which originated in Europe. We used RAPD-PCR to identify a DNA length polymorphism that is diagnostic for the two moth strains. The polymorphism maps to an autosomal locus with codo...
متن کامل